Fig. 2: Release of cfDNA by colorectal cancer cells in culture.
From: Hypoxia differently modulates the release of mitochondrial and nuclear DNA
Culture media and cell pellets from colorectal cell lines (DiFi, HCT116, SW620) were harvested after 6 h of incubation at a different number of cells per well (25,000, 100,000 or 400,000). Extracellular and intracellular DNA of nuclear (a, c, respectively) or mitochondrial origin (b, d, respectively) were measured by qPCR, as described in the Methods section, and estimation was made of the percentage of DNA released in culture medium as compared to intracellular DNA (e) and of MNR (f). Average values with (±) standard deviations are represented, results having been reproduced in four independent experiments, each performed in triplicate. MNR: Mitochondrial-to-nuclear DNA ratio. P values significance: **P < 0.01.
