Fig. 4: In vivo effects of intermittent hypoxia.
From: Hypoxia differently modulates the release of mitochondrial and nuclear DNA
a Flow chart of the in vivo experiment: C57BL/6j mice were pre-exposed during 2 weeks to either room air or intermittent hypoxia, and then injected with 105 TC1 cells. Blood collection was performed 4 weeks after the graft. The concentrations of circulating cfDNA in plasma from mice subjected to normoxia (open boxes) or hypoxia (grey boxes) were evaluated by qPCR by targeting murine KRAS (b) or COX1 (c) genes. This allowed the evaluation of the nuclear DII (d) and the MNR (e). The effect of hypoxia on tumour weight (f) and mouse body weight gain (g) are also presented. The line from end to end of the whisker represents the minimum and maximum values of the data, the line within each box represents the median and the lower and upper boundaries of the box indicate the first and third quartiles, respectively. Significant differences between normoxia and intermittent hypoxia were estimated using the Mann–Whitney test. P values significance: *p < 0.05, **p < 0.005 and ***p < 0.0005. NcfDNA: nuclear cell-free DNA; McfDNA: mitochondrial cell-free DNA; DII: DNA integrity index; MNR: mitochondrial-to-nuclear DNA ratio; dBWT: delta body weight; n.s.: not significant.
