Table 1 List and sequence of human and murine primers used for qPCR assays.
From: Hypoxia differently modulates the release of mitochondrial and nuclear DNA
Human | Primer name | Sens | Sequence | TM (°C) | %GC | Amplicon size (bp) |
|---|---|---|---|---|---|---|
Nuclear DNA | KRAS B2 | Reverse | CCCTGACATACTCCCAAGGA | 59.4 | ||
KRAS B1 | Forward | CCTTGGGTTTCAAGTTATATG | 54 | 67 | ||
KRAS A1 | Forward | GCCTGCTGAAAATGACTGA | 54.5 | 305 | ||
Mitochondrial DNA | MT-COX3 F | Forward | GACCCACCAATCACATGC | 56 | 55.6 | |
MT-COX3 R 67 | Reverse | TGAGAGGGCCCCTGTTAG | 58.2 | 61.1 | 67 | |
MIT-COX3 R 296 | Reverse | CTCAGAAAAATCCTGCGAAGA | 55.9 | 42.9 | 296 |
Mouse | Primer name | Sens | Sequence | TM (°C) | %GC | Amplicon size (bp) |
|---|---|---|---|---|---|---|
Nuclear DNA | MU KRAS 61–146 R | Reverse | GCTTCATTATCCTGCTTCC | 56 | 47.4 | |
MU KRAS 61 R | Forward | TTGATTCCTTGCTAGTTCT | 52 | 36.8 | 61 | |
MU KRAS 146 F | Forward | TTCCCTGGGTTTTGGACTTA | 45 | 146 | ||
Mitochondrial DNA | MU MT COX1 F | Forward | GTCCCACTAATAATCGGAGC | 60 | 50 | |
MU MT COX1 R REV C | Reverse | TGCTTCTACTATTGATGATGC | 58 | 55.5 | 114 |
