Fig. 3: BRD/BET proteins are required for adaptive ErbB3 upregulation following BRAF/MEK inhibitors treatment in BRAF-mutant melanoma.

a Scatter plots with trend lines of BRD2 mRNA versus ERBB3 mRNA z-score data from BRAF-mutant TCGA cutaneous melanoma samples (nâ=â142). b Scatter plots with trend lines of BRD4 mRNA versus ERBB3 representative Western blots of three (nâ=â3) independent experiments for ErbB3, PDGFR-β, and AXL after BRD2 and BRD4 knockdown, and c 24âh of treatment using BRAFi/MEKi (dabrafenib, 50ânM + trametinib, 5ânM) for the BRAF-mutant human melanoma cell lines, 1205Lu and M238. The siRNA scramble control (siCTRL), BETi (PLX51107, 2âÎĵM) single agent, and BRAFi/MEKi/BETi treatments for 24âh were used as controls.