Fig. 2: Differential gene profiling of Hep3B cells with GD treatment. | British Journal of Cancer

Fig. 2: Differential gene profiling of Hep3B cells with GD treatment.

From: Genkwadaphnin inhibits growth and invasion in hepatocellular carcinoma by blocking DHCR24-mediated cholesterol biosynthesis and lipid rafts formation

Fig. 2

GD represses DHCR24 expression and activity. a Hep3B cells were treated with 7.5 μM GD in triplicate for 48 h and the global gene expression change in the GD treatment group and corresponding control group was analysed by microarray assay. GO enrichment analysis (cellular component) and KEGG pathway analysis of the differentially expressed protein-coding genes affected by GD. b Heatmaps exhibit significant differential genes involved in metabolism pathway and the pathways identified in KEGG and GO analyses. c Validation of some differential expressed genes by quantitative real-time PCR analysis in Hep3B and PLC/PRF/5 cells treated with GD at the indicated doses for 48 h. d The effect of GD on DHCR24 protein expression was determined by western blot analysis. Whole-cell lysates were analysed by antibodies against DHCR24 protein with β-actin as loading control. e ELISA analysis of DHCR24 enzyme activity in Hep3B and PLC/PRF/5 cell lines with indicated concentrations of GD treatment. n = 3, means ± SD, *P < 0.05, **P < 0.01, ***P < 0.001 compared with control group.

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