Table 2 The primer sequences used in the quantitative real-time PCR experiments.

From: Genkwadaphnin inhibits growth and invasion in hepatocellular carcinoma by blocking DHCR24-mediated cholesterol biosynthesis and lipid rafts formation

Gene

Forward primer 5′–3′

Reverse primer 5′–3′

DHCR24

GCACAGGCATCGAGTCATCAT

GTGCATCGCACAAAGCTGC

β-Actin

TTGCGTTACACCCTTTCTTG

CACCTTCACCGTTCCAGTTT

PAK1

AGGGGAGTTTACGGGAATGC

TCTTCTGCTCCGACTTAGTGATA

NR2F2

TCATGGGTATCGAGAACATTTGC

TTCAACACAAACAGCTCGCTC

FGFR1

GGCTACAAGGTCCGTTATGCC

GATGCTGCCGTACTCATTCTC

MMP16

AGCACTGGAAGACGGTTGG

CTCCGTTCCGCAGACTGTA

HMGCR

CAAGGAGCATGCAAAGATAATCC

GCCATTACGGTCCCACACA

CYP51A

GCAGGGATGCTTATTGGATTACTC

AGAAGCCCATCCAAGCACTAG

SQLE

GAGATGGAAGAAAGGTGACAGTCA

CACCCGGCTGCAGGAAT

DHCR7

ACGCTACACTCTGCCCACACT

CAAGAGTAAATGCAGCCTAATGACA

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