Fig. 1: Discovery of the CD63–BCAR4 fusion gene in a patient with lung adenocarcinoma.
From: A fusion of CD63–BCAR4 identified in lung adenocarcinoma promotes tumorigenicity and metastasis
a Schematic representations of the wild-type CD63 and BCAR4 genes with breakpoints of the respective exon numbers and CD63–BCAR4 fusion. The sequencing chromatogram shows the breakpoint, confirmed by Sanger sequencing. b Expression of fusion gene of CD63–BCAR4 was confirmed by RT-PCR in the tumour tissue of a positive patient (NCCLUAD-010). c High expression of BCAR4 mRNA in the index case. Quantitative real-time RT-PCR was performed to compare mRNA expression of BCAR4 and CD63 in tumour (T) and adjacent normal (N) tissues from four patients. * Indicates the index case. d Expression of BCAR4 mRNA in lung cancer cell lines showing mutation status of EGFR, KRAS and ALK genes. e Fusion transcripts of BCAR4 that were detected in the patients with lung adenocarcinoma. References were indicated for previously reported fusions.
