Fig. 2: Increased cell proliferation and colony formation by CD63–BCAR4.
From: A fusion of CD63–BCAR4 identified in lung adenocarcinoma promotes tumorigenicity and metastasis
a Relative cell growth (%) of BEAS-2B cells expressing empty vector (Vector) or CD63–BCAR4. Cell proliferation of BEAS-2B (bronchial epithelial cell line) and NCI-H1299 (lung cancer cell line) was measured using the IncuCyte™ Live-Cell Imaging System. b Representative colonies and relative colony-forming activity of CD63–BCAR4 or empty vector-expressing cells. c Representative DNA histograms and bar graph of cell-cycle distribution at the indicated time points after a double-thymidine block. Overexpressed BEAS-2B cells were synchronised in G1 by a double-thymidine block, and then released into the cell cycle for the times indicated. Each bar in the graph indicates the average cell-cycle distribution from triplicated experiments. d Expression levels of cell-cycle-related genes after exogenous expression of CD63–BCAR4 and BCAR4. The relative expression was compared to the control gene GAPDH by using semi-quantitative RT-PCR. Data in this figure are presented as the representative results of three independent experiments. Bar graphs demonstrated the mean ± SD. Statistically significant differences are marked with *(p < 0.05), ** (p < 0.005) and ***(p < 0.0005), respectively.
