Fig. 1: NCB-0846 inhibits TGFβ1-triggered EMT.

a Morphological conversion of A549 (upper panels) and H2228 (lower panels) cells. Following 24-h serum starvation, cells were treated with DMSO alone (control), TGFβ1 (5 ng/mL) and DMSO, TGFβ1 and NCB-0846 (3 µM), or TGFβ1 and NCB-0970 (3 µM) for 48 h and photographed. Scale bar, 100 µm. b Immunoblot analysis of epithelial (E-cadherin) and mesenchymal cell markers (vimentin and N-cadherin) and γ-tubulin (loading control) in A549 (upper panels) and H2228 (lower panels) cells treated as indicated. c Immunofluorescence confocal microscopy of epithelial (E-cadherin and ZO-1) and mesenchymal cell markers (vimentin and fibronectin) in A549 cells treated as indicated. The nuclei were stained with TOTO3 (blue). Scale bar, 20 μm. d Cell migration evaluated by a wound-closure assay. Dotted and solid lines indicate the edges of wounds immediately and 48 h after creation of the wounds, respectively. The width of wounds relative to their baseline (set to one) is shown in the bar graph (right).