Fig. 3: NCB-0846 downregulates the TBFBRI transcript. | British Journal of Cancer

Fig. 3: NCB-0846 downregulates the TBFBRI transcript.

From: Pharmacological blockage of transforming growth factor-β signalling by a Traf2- and Nck-interacting kinase inhibitor, NCB-0846

Fig. 3

a Immunoblot analysis of TGFβRI, TGFβRII and γ-tubulin (loading control) in A549 (left) and H2228 (right) cells treated as indicated. Note that NCB-0846 specifically inhibited the expression of TGFβRI protein. b Relative expression of the TGFBR1 gene (normalised to ACTB) in A549 and H228 cells treated with DMSO alone (control), TGFβ1 (5 ng/mL) and DMSO, TGFβ1 and NCB-0846 (3 µM) or TGFβ1 and NCB-0970 (3 µM) for 48 h. The values for cells treated with DMSO alone are set to one. Error bars represent standard deviations (SD) of triplicate experiments. c Morphology of TGFBR1-knockdown cells. A549 cells transfected with 50 nM control siRNA (siNC) or siRNA to TGFBR1 (siTβRI) were incubated with TGFβ1 (5 ng/mL) for 48 h and photographed. Scale bar, 100 μm. d Immunoblot analysis of TGFβRI, TGFβRII, pSMAD2, pSMAD3, E-cadherin, vimentin, N-cadherin and γ-tubulin (loading control) in A549 cells transfected with control siRNA (siNC) or siRNA to TGFBR1 (siTβRI) and incubated without (−) or with (+) TGFβ1 (5 ng/mL) for 48 h.

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