Fig. 5: ATR or WEE1 inhibition induces DNA damage and replication stress in SLFN11-deficient gemcitabine or etoposide-treated cells.
From: SLFN11 informs on standard of care and novel treatments in a wide range of cancer models
a Staining for DNA (DAPI), pRPA (S4/8) and pH2Ax in DU145 isogenic cells treated for the indicated time points with the designated compounds. The insets show a ×2 magnification. Scale bar, 10 µm. b, c pRPA (S4/8) and pH2Ax mean intensities in DU145 isogenic cells treated with gemcitabine, ATRi (b) or etoposide, ATRi (c) for the indicated time points. The data are presented as mean ± s.e.m. (n = 4) of the control-treated condition. *P < 0.05; **P < 0.01; ***P < 0.001 (paired Student’s t test).
