Fig. 3: IDH2 knockdown further aggravated the radiation-induced oxidative stress in ESCC cells. | British Journal of Cancer

Fig. 3: IDH2 knockdown further aggravated the radiation-induced oxidative stress in ESCC cells.

From: Isocitrate dehydrogenase 2 contributes to radiation resistance of oesophageal squamous cell carcinoma via regulating mitochondrial function and ROS/pAKT signalling

Fig. 3: IDH2 knockdown further aggravated the radiation-induced oxidative stress in ESCC cells.

Transfected Kyse140 and Kyse510 cells were subjected to 0 or 8 Gy radiation at 48 h before detection. Flow cytometric detection of a intracellular ROS and b mitochondria-derived superoxide. Contents of c MDA and d protein carbonyl in Kyse140 and Kyse510 cells. e Detection of 8-OHdG by immunofluorescence staining and quantification analysis of immunofluorescence. Nuclei were counterstained with DAPI. Scale bar = 20 μm. The data shown are the mean ± SD of independent experiments (n = 3). P value was calculated by two-tailed unpaired t test.

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