Fig. 4: CDK4/6 inhibition affetcs the cell cycle and induces apoptosis. | British Journal of Cancer

Fig. 4: CDK4/6 inhibition affetcs the cell cycle and induces apoptosis.

From: CDK4/6 inhibition presents as a therapeutic option for paediatric and adult germ cell tumours and induces cell cycle arrest and apoptosis via canonical and non-canonical mechanisms

Fig. 4: CDK4/6 inhibition affetcs the cell cycle and induces apoptosis.The alternative text for this image may have been generated using AI.

a, b Flow cytometry-based analysis of cell cycle phase distribution (a) and apoptosis rates (b) in GCT cell lines and controls (fibroblasts and Sertoli cells) 16 h after PaRi treatment (10 μM/25 μM). c Phospho-histone H3-positive (pH3+) cells were counted by flow cytometry in solvent and PaRi-treated GCT cells. Differences are given in percentage. d Western blot analysis of PARP (total and cleaved) protein levels in GCT cell lines and MPAF fibroblasts treated for 16 h with PaRi (10 μM/25 μM). Only a long exposure of MPAF enables detection of total PARP. β-Actin was used as housekeeper and for normalisation. e Changes in Caspase-3/7 activity (as fold change) in GCT cell lines after PaRi treatment compared to solvent-treated controls.

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