Fig. 2: JQ1 suppressed expression levels of metastasis genes in OSCC cells.

a JQ1 suppressed the genes involved in metastasis in HOC313 cells. Scatter plot showing the microarray analysis of mRNA expression levels of 159 genes involved in the metastasis of OSCC in HOC313 cells treated with JQ1 (1.0 μM for 48 h) or vehicle. Red dots indicate genes with Z-score ≥2.0 and ratio ≥1.5-fold or Z-score ≤−2.0 and ratio ≤0.66. b JQ1 repressed 15 genes involved in metastasis. The expression levels in HOC313 cells treated with JQ1 (1.0 μM for 48 h) were determined by qRT-PCR. The relative mRNA levels to non-treated cells are shown. The values were normalised to 36B4. Data are mean ± SEM from three independent experiments. P values were calculated using the unpaired two-tailed Student’s t test (*P < 0.05, **P < 0.01, ***P < 0.001). c JQ1 did not affect the BRD4 protein levels. Immunoblotting showing the BRD4 protein levels in HOC313 cells treated with vehicle or JQ1 (1.0 μM for 24 and 48 h).