Fig. 6: CD109/EGFR/STAT3 signalling may response for cell migration, proliferation and maintenance of CSC phenotype. | British Journal of Cancer

Fig. 6: CD109/EGFR/STAT3 signalling may response for cell migration, proliferation and maintenance of CSC phenotype.

From: CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma

Fig. 6: CD109/EGFR/STAT3 signalling may response for cell migration, proliferation and maintenance of CSC phenotype.

Western blotting for EGFR expression and phosphorylation of STAT3 on a panel of human cervical cancer cell lines (a), sorted CD109(−) and CD109( + ) cells in C4-1 and CaSki lines (b), NTC siRNA or CD109 siRNA-knockdown cells in C4-1, CaSki and SiHa lines (c) and CaSki control or Q4-CD109-KO cells, SiHa control or L65-CD109-KO cells (d). Western blotting for phosphorylation of STAT3 on sorted CD109(−) and CD109( + ) cells with/without anti-EGFR treatment (e). Representative images of spheroids formed by sorted CD109( + ) CaSki cells treated w/o EGFR (magnification, ×4, ×10). The bar graph (below) represents the mean number of spheroids from each well under the microscope, and error bars represent SD, *P = 0.0021 (f). Representative images of spheroids formed by NTC siRNA or STAT siRNA-knockdown CaSki CD109( + ) cells (magnification, ×4, ×10). The bar graph represents *P = 0.0030 (g). Western blotting for stem cell markers on sorted CD109(−), NTC siRNA or STAT siRNA-knockdown CD109( + ) CaSki cells (h).

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