Fig. 2: Effect of CDK5RAP3 on EMT and acquisition of CSC phenotypes in gastric epithelial cells. | British Journal of Cancer

Fig. 2: Effect of CDK5RAP3 on EMT and acquisition of CSC phenotypes in gastric epithelial cells.

From: RETRACTED ARTICLE: CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer

Fig. 2: Effect of CDK5RAP3 on EMT and acquisition of CSC phenotypes in gastric epithelial cells.

a Western blot demonstrating levels of CDK5RAP3, CD44, Sox2, Oct4, Nanog and c-Myc in HFE-145 spheroid cells transduced with sh. CDK5RAP3 or sh. Scr. b Photos and graph of HFE-145 cells following transduction sh.CDK5RAP3 or sh.Scr and grown in spheroid formation conditions. c Immunofluorescence of HFE-145 spheroids for DAPI (blue), CD44 (green) and Sox2 (red) following transduction with sh.CDK5RAP3 or sh. Scr. Scale bar 50 μm. d Photos and graphs of migration and invasion assays for HFE-145 cells in monolayer cells and in spheroids transduced with sh.CDK5RAP3 or sh.Scr. e Western blot demonstrating levels of CDK5RAP3, E-cadherin, N-cadherin, Vimentin, Snail, Slug and Zeb1 in HFE-145 spheroid cells transduced with sh.CDK5RAP3 or sh.Scr. f Photos following immunofluorescence staining HFE-145 spheroid cells transduced with sh.CDK5RAP3 or sh.Scr for DAPI (blue), CD44 (Green) and Snail (Red). Scale bar, 50 μm. β-actin was used as a loading control.

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