Fig. 3: Effect of CDK5RAP3 in gastric CSCs. | British Journal of Cancer

Fig. 3: Effect of CDK5RAP3 in gastric CSCs.

From: RETRACTED ARTICLE: CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer

Fig. 3

a Photos and graph of AGS and KATOIII cells transduced with CDK5RAP3 or Vector and grew in spheroid formation conditions. Scale bar, 50 μm. b Western blot analysis and immunofluorescence staining demonstrating levels of CDK5RAP3, CD44, Sox2, Oct4, Nanog and c-Myc in spheroid cells of AGS and KATOIII transduced with CDK5RAP3 or Vector. c Graphs and photos of migration and invasion assays for AGS and KATOIII spheroid cells transduced with CDK5RAP3 or Vector. d Photos and graph of NCI-N87 and SNU668 cells transduced with sh.CDK5RAP3 or sh.Scr and grown in spheroid formation conditions. Scale bar, 50 μm. e Photos and graphs of migration and invasion assays for AGS and KATOIII in spheroids transduced with CDK5RAP3 or Vector. f Photos and graphs of migration and invasion assays for NCI-N87 an dSNU668 in spheroids transduced with sh.CDK5RAP3 or sh.Scr. β-actin was used as a loading control. *p < 0.05 compared to control.

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