Fig. 5: ERK signalling promotes CSC phenotypes and inhibits expression of CDK5RAP3 in gastric cancer cells. | British Journal of Cancer

Fig. 5: ERK signalling promotes CSC phenotypes and inhibits expression of CDK5RAP3 in gastric cancer cells.

From: RETRACTED ARTICLE: CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer

Fig. 5

a Human phospho-kinase antibody array and western blot analysis to determine kinases differentially regulated in KATOIII cells cultured as spheroids vs. monolayers. b Western blot analysis demonstrating levels of CDK5RAP3, p-ERK1/2, ERK2, p-JNK1/2, JUK2, p-p38 and p38 in monolayers and spheroid cells of AGS and KATOIII. c Photos of single cell assay in AGS and KATOIII cells showing diameter of spheroids at selected time points following treatment of ERK inhibitor U0126 or carrier (DMSO) and grown in spheroid formation conditions. d Western blot analysis of AGS and KATOIII gastric cancer spheroid cells treated with U0126 or DMSO in spheroid formation condition for levels of phospho-ERK1/2, ERK2 and CDK5RAP3. e Western blot demonstrating levels of CDK5RAP3, p-ERK1/2, ERK2, p-JUK1/2, JNK2, p-p38 and p38 in spheroids of AGS and KATOIII transduced with CDK5RAP3 or Vector. β-actin was used as a loading control.

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