Fig. 6: ERK-signalling inhibition and CDK5RAP3 negatively regulate EMT and metastasis in gastric CSCs. | British Journal of Cancer

Fig. 6: ERK-signalling inhibition and CDK5RAP3 negatively regulate EMT and metastasis in gastric CSCs.

From: RETRACTED ARTICLE: CDK5RAP3 as tumour suppressor negatively regulates self-renewal and invasion and is regulated by ERK1/2 signalling in human gastric cancer

Fig. 6

a Western blot analysis of AGS and KATOIII spheroid cells treated with U0126 or DMSO in spheroid formation condition for levels of phospho-ERK1/2, ERK2, CDK5RAP3, Snail, Slug and Zeb1. b Photos and graphs of migration and invasion assays for AGS and KATOIII spheroid cells treated with U0126 or DMSO. c Immunofluorescence of AGS and KATOIII spheroid cells for DAPI (blue), CD44 (green) and snail (red) following transduction with sh.Kras or sh. Scr. Scale bar 50 μm. d Graphs of migration and invasion assays for AGS and KATOIII spheroid cells transduced with sh.Kras or sh.Scr. e Western blot analysis and immunofluorescence staining of AGS and KATOIII spheroid cells transduced with CDK5RAP3 or Vector for levels of CDK5RAP3, Snail, Slug and Zeb1. Scale bar 50 μm. β-actin was used as a loading control. *p < 0.05 compared to control.

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