Fig. 4: M2BPGi promoted HCC tumorigenesis through interactions with galectin-3 (Gal-3). | British Journal of Cancer

Fig. 4: M2BPGi promoted HCC tumorigenesis through interactions with galectin-3 (Gal-3).

From: Mac-2-binding protein glycan isomer enhances the aggressiveness of hepatocellular carcinoma by activating mTOR signaling

Fig. 4

a Compared with control, M2BPGi did not enhance the proliferation of HCC cell lines (PLC/PRF/5) transfected with Gal-3 siRNA (n = 6, *p < 0.01). Western blotting validated the suppression of Gal-3 by specific siRNAs. b M2BPGi did not enhance the invasion of HCC cell lines (PLC/PRF/5) transfected with Gal-3 siRNA compared with controls (n = 5, *p < 0.01). c M2BP immunoprecipitation using a Gal-3-specific antibody (Gal-3 IP)-detected M2BP at 75-kDa band in whole-cell lysate. M2BPGi-treated Gal-3 IP samples, but not controls, appeared as a broad M2BP band at 85–97 kDa. d Membranous Gal-3 protein expression in PLC/PRF/5 cells treated with M2BPGi. E cadherin was used as a membrane protein- loading control.

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