Fig. 2: Patient-specific CAF tissue sample characterisation.
From: Cancer-associated fibroblasts mediate cancer progression and remodel the tumouroid stroma
a Origin of samples, including the location of the original cancer mass, tumour type and any additional notes. b 2D cell samples were analysed for fibroblast markers ACTA2 (alpha smooth muscle actin), c S100A4 (fibroblast-specific protein-1), d PDGFRA (platelet-derived growth factor receptor a), e FAP (fibroblast-activating protein), f IL-6 (interleukin-6) and g P4HA1 (prolyl-4-hydroxylase). The value shown is normalised to HPRT1mRNA levels (mean ± SEM) with n = 3 and three technical repeats. One-way ANOVA with Dunnet’s post hoc for ACTA2, S100A4, FAP, PDGFRA and IL-6 and Kruskal–Wallis with Dunn’s multiple-comparison test P values for P4HA1, with values 0.05 = *, 0.005 = **, 0.0005 = *** and 0.00005 = **** with DOF for the first five genes all=20 and F value for ACTA2 = 92.1, S100A4 = 136.7, FAP = 31.83, PDGFRA = 16.2 and IL-6 = 13.76. h Western blot of α-SMA protein expression within 2D monolayers of cells with LC = loading control beta-tubulin. i, j Visualisation of vimentin expression of HDF and CAFs, respectively, when cultured in 3D with scale bar = 100 µm for both images and green = vimentin and blue = DAPI.
