Fig. 2: Illustration of dEGFR39 distribution in the three reactions and output data from digital PCR in the form of a 2D histogram. | British Journal of Cancer

Fig. 2: Illustration of dEGFR39 distribution in the three reactions and output data from digital PCR in the form of a 2D histogram.

From: Plasma-based early screening and monitoring of EGFR mutations in NSCLC patients by a 3-color digital PCR assay

Fig. 2

a 19DelWT molecules are double positive (CY5+/HEX+), and the rest of the signals in the HEX and CY5 channels are generated by 19DelMU (CY5−/HEX+) and S768IMU (CY5+/HEX−), respectively. The signals in the FAM channel are generated by L858RMU. b 20insWT molecules are double positive (CY5+/HEX+), and the rest of the signals in the HEX and CY5 channels are generated by G719XMU (CY5−/HEX+) and 20insMU (CY5+/HEX−), respectively. c The blue and green dots represent the signal of T790M and C797S in trans configuration with T790M, respectively. The double positive represents C797S in cis configuration with T790M.

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