Fig. 3: CXCL11 promotes angiogenesis by promoting EGF expression in ATC. | British Journal of Cancer

Fig. 3: CXCL11 promotes angiogenesis by promoting EGF expression in ATC.

From: The role of anlotinib-mediated EGFR blockade in a positive feedback loop of CXCL11-EGF-EGFR signalling in anaplastic thyroid cancer angiogenesis

Fig. 3

a The average expression score of CXCL11 in the tumour was higher than that in matched peritumour from ATC tissue microarray. b rhCXCL11 (100 ng/ml for 2 h) could improve the abilities of tubule formation, migration and proliferation of HUVEC. c Supernatants from CXCL11 knockdown ATC cells could decrease the tubule formation and migration of HUVEC. d Supernatants from CXCL11 overexpression ATC cells could increase the tubule formation and migration of HUVEC. e Quantification of up- and downregulated angiogenetic genes by PCR array with HUVEC treated by rhCXCL11 (100 ng/ml for 2 h) against negative control. f Expression of EGF was detected when HUVEC treated with rhCXCL11 under CXCR3 or CXCR7 knockdown. g, h HUVEC was cultured with supernatants from CAL-62 and KHM-5M cells overexpressing CXCL11. All data are obtained from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.

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