Fig. 1: MCL-1 protein expression correlates with USP9X expression in oral cell lines and tissues. | British Journal of Cancer

Fig. 1: MCL-1 protein expression correlates with USP9X expression in oral cell lines and tissues.

From: Elevated USP9X drives early-to-late-stage oral tumorigenesis via stabilisation of anti-apoptotic MCL-1 protein and impacts outcome in oral cancers

Fig. 1

a The basal-level expression of USP9X, MCL-1, BCL-2, BCL-XL and actin was assessed by western blotting in three oral cancer cell lines. The blots are representative of three experiments. The data points in the graph are mean ± SEM of three independent repeats and represent a correlation between normalised expression of MCL-1 and USP9X proteins. b The expression of MCL-1 and USP9X proteins was assessed by western blotting in a set of eight adjacent normal-tumour paired samples of oral cancer patients. Densitometric analysis was performed, and the normalised expression of both proteins in paired normal and tumour tissues is represented as mean + SEM. c The expression of MCL-1 and USP9X proteins was assessed by IHC in the oral tissue samples of NOM, OL, OSMF and OSCC and represented in terms of H Score. d The expression of MCL-1 and USP9X proteins in oral tissue samples of NOM, OL, OSMF and OSCC was determined by IHC. The images are the best representatives of the oral histopathological type studied for the individual proteins (scale bar: 200 μm, inset scale bar: 50 μm). Correlation analysis was performed between MCL-1 and USP9X protein expression in terms of H Score. ns not significant, r Pearson’s correlation coefficient.

Back to article page