Fig. 4: ASA alters the chromatin accessibility of pCSCs in ESCC cells.

a Schematic diagram of acetylation of serine hydroxyl groups on histone by ASA. b Immunoblotting for cleaved caspase-3, Ac-H3K9 and Ac-H3K27 in SCs in the presence of ASA. c MA plots showing log 2 fold change of chromosome-accessibility peaks of ATAC-seq analysis in KYSE410 between ASA or DMSO treatment. d Venn diagram for genes with altered chromatin accessibility upon ASA-treatment in KYSE410-SCs and WCs. e KEGG pathway analysis among genes altered by ASA for chromatin accessibility in KYSE410-SCs. f Genome browser image showing ATAC-seq analysis of CCND3 with a grey box indicating changed chromatin accessibility upon ASA treatment.