Fig. 5: ASA induces cell death in pCSCs of ESCC dependent on Bim up-regulation. | British Journal of Cancer

Fig. 5: ASA induces cell death in pCSCs of ESCC dependent on Bim up-regulation.

From: Aspirin enhances the therapeutic efficacy of cisplatin in oesophageal squamous cell carcinoma by inhibition of putative cancer stem cells

Fig. 5

a qRT-PCR results showing the expression of indicated genes in SCs upon treatment with 5 mM ASA (n = 3, normalized to GAPDH expression). b Immunoblotting for Bim in WCs and SCs in the presence of ASA. c Genome browser image showing ATAC-seq analysis of BCL2L11 with a grey box indicating changed chromatin accessibility upon ASA-treatment. d Western blot analysis confirming siRNA-mediated Bim knockdown in KYSE180 and KYSE410 cells. e, f Representative images of Annexin V assay and quantification show the percentage of apoptotic cells of KYSE180 (e) and KYSE410 (f) cells following 24 h treatment with DDP (5 μg/mL) or combined with ASA (5 mM) after knocking down Bim expression (n = 3). g Representative images of IHC staining against Bim on xenograft tumour sections following treatment with ASA or combined with DDP. Scale bars, 100 μm. In all the panels, error bars indicate means ± SD; *p < 0.05, ***p < 0.001 and  ****p < 0.0001; n.s. not significant. Student’s t test.

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