Fig. 4 | British Journal of Cancer

Fig. 4

From: Whole-genome sequencing facilitates patient-specific quantitative PCR-based minimal residual disease monitoring in acute lymphoblastic leukaemia, neuroblastoma and Ewing sarcoma

Fig. 4

Relative expression of mRNA transcripts relative to β2M in healthy bone marrow (BM) cells spiked with a HR-NB and b EWS patient-derived xenograft cells. Cell spike-in ranges are 10,000 (10−1)–10 (10−4) HR-NB or EWS cells in bone marrow control cells. The MRD values were plotted against sample serial dilutions. Each dot represents average transcript levels in one patient from triplicate experiments. Error bars indicate mean ± standard deviation (SD). c and d Pearson’s correlation plots of DNA and RNA-based MRD analysis in HR-NB (c) and EWS (d) patient samples. TH expression in HR-NB and EWSR1-FLI1 expression in EWS patients were compared against patient-specific WGS-MRD assays. Dotted lines indicate positive but non-quantitative (PNQ) MRD as assigned according to EuroMRD guidelines.

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