Fig. 3: Technical challenges in PVT1 transcript quantification and silencing assays. | British Journal of Cancer

Fig. 3: Technical challenges in PVT1 transcript quantification and silencing assays.

From: Unravelling similarities and differences in the role of circular and linear PVT1 in cancer and human disease

Fig. 3: Technical challenges in PVT1 transcript quantification and silencing assays.

a Convergent primers (yellow arrows) designed on lncPVT1 exon 2 give rise to PCR products from both linear and circular isoforms (grey dashed line), whereas divergent primers (blue arrows) amplify a specific PCR product from circPVT1. b A siRNA designed on lncPVT1 exon 2 (black line) silences both lncPVT1 and circPVT1. The specific silencing of circPVT1 can be achieved using a siRNA targeting its back-splicing junction (green line). Grey arrows = transcript silencing.

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