Fig. 3: Novel ZNF394-BRAF fusion gene identified in patient P2847 via a tandem duplication on chromosome 7.

The tumour specimen in this case was bi-phasic with both high- and low-grade components (a). The high-grade tissue was selected for whole-genome sequencing. The tandem duplication apposed exons 1–2 of ZNF394 with exons 10–18 of the BRAF oncogene preserving the BRAF kinase domain consistent with functional validity (b).