Fig. 2: Effect of 5-ALA on intra-cellular mitochondrial ROS production and MMP after RT in PC-3 cells. | British Journal of Cancer

Fig. 2: Effect of 5-ALA on intra-cellular mitochondrial ROS production and MMP after RT in PC-3 cells.

From: 5-Aminolevulinic acid overcomes hypoxia-induced radiation resistance by enhancing mitochondrial reactive oxygen species production in prostate cancer cells

Fig. 2: Effect of 5-ALA on intra-cellular mitochondrial ROS production and MMP after RT in PC-3 cells.

a ROS production in mitochondria after different treatments performed using MitoSOX. b Mitochondrial membrane potential performed by JC-1 staining. Merging of J-aggregates (red) and J-monomers (green) has been shown. Apoptotic cells and depolarised mitochondria are detected by the green color. a, b Images were taken at 1, 6, and 12 h after radiation. c Relative fluorescence intensity of MitoSOX. Statistical analysis; by Student’s t-test (mean ± SEM, n = 5). d Relative fluorescence intensity of JC-1 staining (red color/green color intensity). Statistical analysis; by Student’s t-test (mean ± SEM, n = 5). *p < 0.05, **p < 0.001. IR ionising radiation.

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