Fig. 5: Cariporide and eslicarbazepine acetate have no effect on tumour [Na⁺] in MDA-MB-231 xenografts.

Rag2^−/− Il2rg^−/− mice bearing MDA-MB-231 tumours were randomised to receive either cariporide (3 mg/kg) vs vehicle i.p. once daily or eslicarbazepine acetate (ESL, 200 mg/kg) vs vehicle p.o. once daily commencing 1 week post xenograft implantation. Representative ¹H TurboRARE and ¹H/²³Na 2D cartesian (merge) images for (a) ESL vs vehicle (e) cariporide vs vehicle at week 4 post implant; regions of interest (ROIs) for tumour (white dash) and NaCl phantom (50 mM, yellow dash) are annotated. Mean (b, f) and maximum (c, g) ²³Na signals from tumour ROIs (normalised to phantom to provide a concentration measurement) were measured at weeks 2, 3 and 4 post xenograft implantation (cariporide group: n = 5 per group per timepoint. ESL vehicle group: week 2, n = 3; week 3, n = 3; week 4, n = 4. ESL treatment group: week 2, n = 4; week 3, n = 4; week 4, n = 5). The tumour growth rate was measured by multislice ¹H TurboRARE imaging (d, ESL. Vehicle, n = 4; treatment group, n = 5. h cariporide. Vehicle week 4, n = 4; all other data points n = 5). Data represent group mean ± SEM. P-values for significant differences between groups calculated using an unpaired, two-tailed Student’s t test.