Table 2 In vitro culture of CTCs isolated from blood samples of patients with cancer.
Cancer type | Isolation methods | Culturing conditions | Time in culture | Ref |
|---|---|---|---|---|
Breast cancer | Multiparametric FACS analysis | First 7 days: DMEM/F12 with 5 mg/ml insulin, 0.5 mg/ml hydrocortisone, 2% B27, 20 ng/ml EGF, and 20 ng/ml FGF-2. From day 8: EpiCult-C medium with 10% FBS and 1% penicillin/streptomycin at 37 °C, 5% CO2. | Short term | [19] |
Breast cancer | CTC-iChip | Serum-free medium supplemented with EGF and bFGF (8) in hypoxic conditions (4% O2). | Long term | [21] |
Breast cancer | Laser-ablated microwell-based method | High-glucose DMEM with 10% FBS and 1% penicillin–streptomycin, at 37 °C in 5% (v/v) CO2 and 1% O2 in humidified conditions. | Short term | |
Breast cancer | Ficoll-Paque density gradient and CD45 RosetteSep negative selection | RPMI-1640, 10% FCS) 1% penicillin–streptomycin, 1% l-glutamine, 1% insulin-transferrin-selenium-A supplement (100×) liquid, 10 ng/ml FGF-2, 50 ng/ ml EGF, 0.1 lg/ml hydrocortisone, and 0.2 lg/ml cholera toxin, at 37 °C, 5% CO2. | Long term | [91] |
Colon cancer | Ficoll-Paque density gradient and CD45 RosetteSep negative selection | RPMI-1640 with 2% FBS, EGF and FGF-2, insulin-transferrin-selenium supplement, l-glutamine. | Long term | |
Non-small cell lung cancer | Herringbone-chip | 7 days on a chip in a mixture of cancer-associated fibroblasts-GFP, collagen I and Matrigel, at 37 °C, 7.5% CO2, Then in culture plate with RPMI complete medium (10% FBS and 1% penicillin/streptomycin). | Short term | [92] |
Small cell lung cancer | Ficoll-Hypaque density gradient | Serum-free RPMI-1640 medium supplemented with insulin, IGF-1, transferrin. | Short term | [32] |
Prostate cancer | Ficoll-Paque density gradient and CD45 RosetteSep negative selection | DMEM/F12 medium, with EGF, bFGF, FGF10, R-spondin 1, DHT, B27, nicotinamide, A83-01, SB202190 and Y27632, in Matrigel. | Long term | [33] |
