Fig. 1: T cell proximity and density score analyses in colorectal cancer.

The figure shows analysis steps for one example tumour core from the tumour centre (CT) and the invasive margin (IM). Tumour cores stained with CD3 and CD8 (a) and corresponding phenotyping maps for T cells, tumour cells and other cells (b). G-cross (G_{tumour:T cell}) function curves, representing the likelihood of any tumour cell in the sample being co-located with at least one CD3⁺/CD8⁺ T cell within a radius r (c). Calculation charts for T cell proximity score (d) and for T cell density score (e). Respective example images for two remaining cores of the same sample are shown in Fig. S2.