Fig. 4: FoxP3+/CD8+ TIL ratio of primary lesions and lymph nodes.

a Dot plot showing that the FoxP3+/CD8+ TIL ratio (FCR) is higher in metastatic lymph nodes than in primary lesions. ***P < 0.001. b Dot plot showing that the FCR in TFLN is higher in patients with ICC and lymph node metastasis than in those without lymph node metastasis. One FCR value in patients with ICC and lymph node metastasis was not shown in this figure because of outlier. TFLN tumour-free lymph node, **P < 0.01. c Dot plot showing the relatively strong correlation between the FCR in TFLN and that in the primary lesions (P = 0.01, rho = 0.478, Spearman’s rank correlation coefficient). d Dot plot showing the relatively strong correlation between the FCR in TFLN and the number of metastatic lymph nodes (P = 0.001, rho = 0.571, Spearman’s rank correlation coefficient).