Fig. 5: Cytokines expression levels in SCLC. | British Journal of Cancer

Fig. 5: Cytokines expression levels in SCLC.

From: Topoisomerase I poison-triggered immune gene activation is markedly reduced in human small-cell lung cancers by impairment of the cGAS/STING pathway

Fig. 5

Expression level of a panel of cytokine genes in (a) H209, (b) H889 and (c) DMS114 cell line in response to CPT and LMP776. Data are represented as mean of two biological replicates ± SEM; P values are indicated on the top of each bar and they are calculated as one-tailed ratio paired t test; same experimental condition of Fig. 1a. For (a–c), nd = not determined value because under detection level. Western blot of (d) STING protein and (e) cGAS protein level in several cell types. TOP1 protein levels are also shown. Red ponceau as loading control is in Supplementary Fig. S6. Fold change (FC) represent quantification values compared to HeLa cells (normalised on red ponceau). mRNA quantification of (f) STING and (g) cGAS gene level in HeLa and lung cancer cell lines. Expression levels were determined by qRT-PCR and normalised on cytochrome c transcript.

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