Fig. 2: Arachidonic acid-induced transendothelial invasion requires pAkt dependent non-canonical pEphA2S897 signalling. | British Journal of Cancer

Fig. 2: Arachidonic acid-induced transendothelial invasion requires pAkt dependent non-canonical pEphA2S897 signalling.

From: Non-canonical EphA2 activation underpins PTEN-mediated metastatic migration and poor clinical outcome in prostate cancer

Fig. 2

a Real-time invasion assay towards a 20 µM AA substrate at 37 °C 5% CO2 through a Matrigel and BMEC barrier. b Diagrammatic representation of the non-canonical pathway initiated by arachidonic acid. c Western blot images showing temporal alteration in expression of total pEphA2S897, pEphA2Y772, EphA2, pAktS473 and Akt after stimulation with 20 µM AA. d Temporal expression of pEphA2Y772 (canonical signalling) and pEphA2S897 (non-canonical signalling) in PTEN− and PTEN+ PC3 cells after stimulation with 20 µM AA. e Endpoint invasion assay measuring invasion of wild-type PC3-PTEN− or PC3-PTEN+ cells through a Matrigel and BMEC barrier towards 20 µM AA. Fluorescence was captured after 18-h incubation at 37 °C, 5% CO2.

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