Fig. 2: Tumours promote and support the expansion of splenic MDSC populations via Treg-independent mechanisms.
From: Treg-driven tumour control by PI3Kδ inhibition limits myeloid-derived suppressor cell expansion
a Tumour weight at day 21 post tumour injection from WT mice treated with either vehicle or PI-3065 or DEREG mice treated with DT (8–10 mice/group). The number of splenic (b) PMN-MDSCs and (c) M-MDSCs from WT mice treated with either vehicle or PI-3065 or DEREG mice treated with DT (3–10 mice/group). The total number of (d) PMN-MDSCs and (e) M-MDSCs in the spleens of tumour-bearing or tumour-naive nude mice at day 28 post tumour injection (4–6 mice/group). f Schematic illustrating the surgical resection time-course utilised. Total numbers of (g) PMN-MDSC and (h) M-MDSC in either tumour-bearing mice at day 21 and day 28 post tumour injection or at the equivalent day’s post tumour resection (3–6 mice/group). All data are displayed as the mean ± SEM. Statistical significance was determined by Kruskal–Wallis test (a–c, g, h) and by unpaired t test (d, e) (*P ≤ 0.05, ***P ≤ 0.001).
