Fig. 1: Integrative Genomic Profiling (IGP) strategy and study design.

a IGP strategy: three different specimens were analysed using three NGS panels for genomic characterisation in each patient; the target regions are summarised (detailed in Supplementary Methods). Selected personalised biomarkers were later interrogated in serial liquid biopsies using a targeted method. b Study design: after the recruitment of candidate patients who fulfilled the inclusion criteria, specimens for IGP were collected. Once RT treatment was initiated, liquid biopsies were performed before and after each session and, also, periodically during the follow-up for further targeted ctDNA analysis. In addition, all the available clinical information, as medical records, patient status and results from diagnostic imaging tests, was gathered.