Fig. 3: Influence of L1 knockdown on Huh7 cells.
a Western blot confirming knockdown of L1 ORF1p in L1KD cells expressing L1-specific shRNA versus cells expressing non-targeting shRNA (NT) and wild-type (WT) cells. GAPDH is used as a loading control. b Graph representing cell migration measured by the closure of scratch wound using automated incucyte system. Values represent combination of three independent experiments. *P ≤ 0.05 for L1KD versus NT and WT from 39 h onwards, two-way ANOVA. c Graph representing number of cells invaded through Boyden chamber in 72 h. Data show combination of 3 independent repeats. **P < 0.01, one-way ANOVA with multiple comparisons. d Graph represents tumour growth upon injection of luciferase labelled Huh7-NT and Huh7-L1KD cells when injected intrahepatically in NSG mice (n = 4) per group, as monitored by IVIS imaging. Sample number was decided based on our previous experience of 100% engraftment. Mice were randomly selected to put in the two groups. *P < 0.05, unpaired t test. e Graph represents ex vivo liver signal in the two groups. f Representative images of tumours developed in the liver visualised by IHC using anti-luciferase antibody. Background liver showing minimal positivity. Scale bar = 4 mm. g Ex vivo images of harvested organs (L lung, K kidney, S spleen) in the indicated groups as visualised by IVIS imager. h Luciferase IHC in the lung of indicated groups to identify metastatic cells.
