Fig. 1: Different cell culture methods used in drug testing.
From: Head and neck cancer patient-derived tumouroid cultures: opportunities and challenges
1a Monolayer monoculture, 1b monolayer mixed culture (e.g.: fibroblasts, mesenchymal stem cells), 2a monoculture spheroids adherent to the flask, 2b mixed culture spheroids adherent to the flask, 2c spheroids in hanging drop method, 2d spheroids suspended in a medium using 3D matrix e.g.: agarose, 2e spheroids suspended in a medium using low attachment U bottom plates, 2f spheroids in stirred systems, such as bioreactors, 3a and 3b Patient-derived tumouroids: Single cells derived from patient tumour samples and suspended in 3D matrixes such as Matrigel or Cultrex BME2.
