Fig. 1: Construction of HER2-specific CAR in primary human peritoneal macrophages. | British Journal of Cancer

Fig. 1: Construction of HER2-specific CAR in primary human peritoneal macrophages.

From: Efficacy evaluation of chimeric antigen receptor-modified human peritoneal macrophages in the treatment of gastric cancer

Fig. 1: Construction of HER2-specific CAR in primary human peritoneal macrophages.The alternative text for this image may have been generated using AI.

a Schematic diagram of experimental design. b Flow cytometry analysis of human primary peritoneal macrophages marker CD11b and CD68. c Construction of chimeric antigen receptor peritoneal macrophages targeting HER2. d Detection of GFP expression in peritoneal macrophages transfected with CAR plasmid by fluorescence microscopy and flow cytometry. e Flow cytometry analysis of the binding of PE-conjugated HER2 protein with HER2-specific scFv on the surface of CAR-PMs. f Phosphorylation levels of downstream transcription factors Syk, Lyn, AKT of CAR-modified PMs and control cells after HER2 protein stimulation for 48 h.

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