Fig. 2: MiR-662 expression in BC subtypes and miR-662 overexpression effect on BC cell proliferation and migration properties.

a Evaluation by real-time qPCR of miR-662 expression in human BC cell lines (T-47D, MCF7, ZR-75-1, BT-474, SK-BR3, Hs-578T, MDA-MB-231) that belong to luminal, TN or HER + BC subtypes. Relative expressions were obtained by comparing ΔCTs for miR-662 amplification to ΔCTs of U6:SNO234 housekeeping genes. b MiR-662 expressions (log₂ Read Count) retrieved from CCLE database of BC cell lines which belong to luminal, TN and HER + BC subtypes. c Evaluation by real-time qPCR of miR-662 basal expression in human normal cell lines (MCF-10A, HMEC-1), BC cell lines (MDA-MB-231 and its engineered luc2-positive subpopulation, NW1), and an engineered murine BC cell line (4T1-luc2). Relative expressions were obtained by comparing ΔCTs for miR-662 amplification to ΔCTs of U6 as housekeeping gene. d Proliferation assay. MiR-662 overexpression promoted cell proliferation in MDA-MB-231 cells compared to control. The significance is observed starting by day 3. e Transwell migration assay. MiR-662 overexpression promoted cell migration across the membrane in MDA-MB-231 cells compared to control. Migrated cells have been manually counted on the entire surface of the membrane. Representative images of haematoxylin/eosin-stained control and miR-662-overexpressing cells migrated through the membrane are shown. Means of three independent experiments ± SEM were shown for all experiments, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.