Fig. 2: The expression of NDUFS1 is reduced in GC cell lines and is mainly located in mitochondria.

a WB analysis of NDUFS1 in GC cell lines and normal gastric epithelial cell lines. Representative WB images (upper) and relative protein level (lower) of NDUFS1 in GC cell lines (GES-1 normal gastric epithelial cell line served as a control; with 1 being the value for GES-1) were shown. *P < 0.05, ANOVA with LSD post hoc multiple comparisons, n = 6 per group. b WB analysis of NDUFS1 in mitochondria of GC cell lines and normal gastric epithelial cell line. Representative WB images (upper) and relative protein level (lower) of NDUFS1 in GC cell lines (GES-1 normal gastric epithelial cell line served as a control; with 1 being the value for GES-1) were shown. *P < 0.05, ANOVA with LSD post hoc multiple comparisons, n = 6 per group. c Representative images of immunofluorescence colocalization of NDUFS1 (green) and TOM20 (red) in GC cell lines and normal gastric epithelial cell lines. Scale bars = 10 μm. d WB analysis of the overexpression or knockdown efficiency of NDUFS1 in GC cells. Representative WB images (upper) and relative protein level (lower) of NDUFS1 in MKN45-NDUFS1 (MKN45-mock served as a control; with 1 being the value for MKN45-mock) and N87-shNDUFS1 (N87-shcontrol served as a control; with 1 being the value for N87-shcontrol) GC cells were shown. *P < 0.05, Student’s t test, n = 6 per group. e WB analysis of NDUFS1 in mitochondria of above cells. Representative WB images (upper) and relative protein level (lower) of NDUFS1 in MKN45-NDUFS1 (MKN45-mock served as a control; with 1 being the value for MKN45-mock) and N87-shNDUFS1 (N87-shcontrol served as a control; with 1 being the value for N87-shcontrol) GC cells were shown. *P < 0.05, Student’s t test, n = 6 per group.