Fig. 1: High abundance of miRNAs containing the key seed region AAGUGC in malignant GCT clinical samples and cell lines. | British Journal of Cancer

Fig. 1: High abundance of miRNAs containing the key seed region AAGUGC in malignant GCT clinical samples and cell lines.

From: Targeting oncogenic microRNAs from the miR-371~373 and miR-302/367 clusters in malignant germ cell tumours causes growth inhibition through cell cycle disruption

Fig. 1

a AAGUGC seed intensity in clinical samples and malignant GCT cell lines. Boxplot of summated miRNA microarray normalised log2 intensity ratios for the 12 miRNAs on the array containing the 2-7nt seed region AAGUGC. Shown are clinical samples, namely normal gonadal controls (n = 8, green), teratomas (n = 5, brown), seminoma (Sem) (n = 13, blue), yolk sac tumour (YST) (n = 12, yellow), embryonal carcinoma (EC) (n = 3, red) and malignant GCT (MGCT) cell lines (n = 6, grey hatched). b–d Relative qRT-PCR expression of b miR-372-3p, c miR-302a-3p, and d miR-519b-3p in testicular and ovarian controls (green), malignant GCT cell lines [blue = Sem, yellow = YST, red = EC, purple = choriocarcinoma (CHC); all hatched], non-GCT cell lines (white hatched), and Universal Reference (Univ Ref) RNA (white dotted—composed of equal quantities of total RNA from 10 human cell lines, including one EC cell line, hence some intermediate expression). e, f Relative contribution of each of the three AAGUGC seed-containing miRNA clusters (miR-371~373, miR-302/367, and C19MC) to overall seed density in seminoma (Sem; left), yolk sac tumour (YST; centre) and embryonal carcinoma (EC; right) cell lines by miRNA microarray (e, left) and qRT-PCR (f, right). Dark grey = miR-371~373, black = miR-302/367, light grey = C19MC.

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