Fig. 4: Metascape pathway analysis of miR-302-SFI effects in malignant GCT cells and corroboration in clinical samples. | British Journal of Cancer

Fig. 4: Metascape pathway analysis of miR-302-SFI effects in malignant GCT cells and corroboration in clinical samples.

From: Targeting oncogenic microRNAs from the miR-371~373 and miR-302/367 clusters in malignant germ cell tumours causes growth inhibition through cell cycle disruption

Fig. 4

a–d Metascape pathway analysis was undertaken for de-repressed mRNAs containing the seed complementary region (SCR) GCACTT, complementary to the 2-7nt AAGUGC miRNA seed of miR-302a-d-3p, and the top-ranking 20 pathways/terms listed in bar plots with significance values. a TCam-2 (Sem; n = 881 genes), b 1411H (YST; 775 genes), c Venn diagram showing there are 188 overlapping common de-repressed mRNA targets between TCam-2 and 1411H, d The common mRNA targets for the cell lines TCam-2 and 1411H, e Corroboration in malignant GCT clinical samples. Metascape pathway analysis for corresponding downregulated (repressed) genes (n = 362) derived from clinical malignant GCT cases. f Associated network analysis derived from data in (e). Key: colour-coding for the three recurring Metascape pathways, namely molecular signal transduction through Rho-GTPases (highlighted in pink), vesicle organisation/transport (light blue), and cell cycle regulation/division (purple). All related GO terms are highlighted, e.g., for vesicle organisation/transport, relevant GO pathways were identified by searching for ‘vesicle’ in the main and related ‘child’ GO terms, and highlighted accordingly.

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