Fig. 1: Multispectral imaging of immune cell infiltration in tumour front and centre of CRC study patients. | British Journal of Cancer

Fig. 1: Multispectral imaging of immune cell infiltration in tumour front and centre of CRC study patients.

From: Opposing roles by KRAS and BRAF mutation on immune cell infiltration in colorectal cancer – possible implications for immunotherapy

Fig. 1

Shown are high-magnification areas (20x) of representative multiplex IHC stains from (a) a TMA (1 mm core) taken at the tumour front and (b) a TMA (1 mm core) taken at the tumour centre. Left panel displays multiplex IHC images after spectral unmixing, of cytokeratin (magenta), CD8+ cytoxic T cells (red), T-bet+ Th1 cells (white), FoxP3+ T regulatory cells (orange), CD20+ B cells (yellow), and CD68+ macrophages (cyan). Right panel displays the same multiplex IHC but with tumour tissue segmentation map of stromal area (blue), tumour epithelial area (magenta), tumour debris (yellow) and no tissue (brown), and the cell phenotypes from which data was collected highlighted using the above described colours.

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