Fig. 6: TTFields and CUSP9v3 combined have enhanced anti-migratory activity.

U251 (a), PC128 (b) and PC40 (c) cells were treated for 24 h as indicated. Afterwards, the cells were enzymatically detached and seeded on 96-well plates followed by sequential microscopic imaging (magnification, × 10) over a total period of 24 h. Wind-rose plots displaying the paths of 15 single cells per treatment condition during the 24 h observation period. The tracks were aligned to start from the same initial position to facilitate comparison. d–f Total distance of 45 cells each tracked within 24 h per treatment condition. Columns, mean; bars, SD. Representative microphotographs of U251 (g) and PC40 (h) cells that were treated for 24 h as indicated followed by inflicting scratches across the subconfluent monolayers of these cells. Magnification, × 10. U251 (i), PC38 (j) and PC40 (k) cells treated as described for (g, h). Data are presented as mean and SD. *p < 0.05, **p < 0.01, ***p < 0.005.