Fig. 5: Re-expression of SHPRH reduces tumor formation, in vivo. | British Journal of Cancer

Fig. 5: Re-expression of SHPRH reduces tumor formation, in vivo.

From: Integrative genomics identifies SHPRH as a tumor suppressor gene in lung adenocarcinoma that regulates DNA damage response

Fig. 5: Re-expression of SHPRH reduces tumor formation, in vivo.

a Tumor volume and b weight measurements of NCI-H1395SHPRH-DEL and NCI-H2009SHPRH-E41* TetO GFP and SHPRH cells subcutaneously injected in alternating flanks of NRG mice. Mice were kept on either a normal or dox diet. For normal diet, N = 6 mice for NCI-H1395 and N = 7 mice for NCI-H2009. For dox diet, N = 10 mice for NCI-H1395 and N = 11 mice for NCI-H2009. Mean ± SEM are shown. Results from Student’s paired t test are indicated, where *p ≤ 0.05 and **p ≤ 0.01. c Representative images of NCI- H1395SHPRH-DEL and NCI- H2009SHPRH-E41* TetO GFP and SHPRH tumors harvested from mice on a dox diet to demonstrate the smaller size of SHPRH-expressing tumors. See Supplementary Figs. 6 and 7 for images of all tumors and Western blot validation of SHPRH and GFP expression.

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