Fig. 6: NLGN4X and VBP1 expression correlate in melanoma patients and increase patient survival time.
From: Late stage melanoma is hallmarked by low NLGN4X expression leading to HIF1A accumulation
a Overall scoring distribution for NLGN4X staining intensity (NL4 int.) in samples distributed according to their ulceration status. Scores of 0 and 1 were regarded as ‘low’ expression and are shown in blue colours, whereas scores of 2 and 3 were regarded as ‘high’ expression and are shown in red colours. Ulceration status is plotted on the x-axis: negative ulceration status (Ulc. No) and positive ulceration status (Ulc. Yes). Staining was evaluated by grouping samples into high (red) and low (blue) NLGN4X expression and performing Fisher’s Exact Test (P values indicated in the graph). b Breslow Depth in samples of high (n = 26) and low (n = 42) NLGN4X staining intensity (NLGN4X stain. intensity). Data presented as mean (± SEM), independent (unpaired) t-test was used for statistical comparisons. c Kaplan Meier plot of patients with low (n = 48) and high (n = 30) NLGN4X expression according to antibody staining intensity. The P value of the log-rank test is shown. d Correlation of NLGN4X and VBP1 histologic staining intensity (n = 50). The correlation coefficient (R) and the P value of the Pearson correlation analysis is shown. e Representative immunofluorescence stain of a human melanoma tissue section for NLGN4X and VBP1 expression. f Kaplan Meier plot (left) of patients divided into low (n = 168) and high (n = 167) risk groups based on their NLGN4X and VBP1 gene expression with corresponding Box plots (right) displaying the expression of the respected genes within the risk groups. The P value of the Log Rank test is presented within the survival plot. Independent (unpaired) t-test was used for statistical comparisons of gene expressions. Data was acquired from the TCGA database. *** = P < 0.001.
