Fig. 3: ONC212 inhibits UM cell migration. | British Journal of Cancer

Fig. 3: ONC212 inhibits UM cell migration.

From: Imipridones inhibit tumor growth and improve survival in an orthotopic liver metastasis mouse model of human uveal melanoma

Fig. 3

a In vitro cell migration assay with ONC212 treatment (0.2 µM) of UM cell lines (OMM1, OMM2.5, MEL20-06-039, MM28, MP65, MP41, and MP46). An average of five fields of cells/filter were counted under a microscope with ×40 magnification. The average number of cells counted/field were obtained in two independent experiments and the mean ± SD of these average cell counts/field were plotted as bar graphs. p values were calculated by comparing untreated vs. ONC212 treated cells (*p < 0.05; **p < 0.01; ***p < 0.001). b Western blots showing the changes in cell migration markers, β1-integrin, and F-actin upon ONC212 treatment in OMM1, MEL20-06-039, MM28 and MP46 cell lines (top panels), and corresponding quantitation of these proteins normalized with β-actin loading control (bottom panels; *p < 0.05; **p < 0.01; ***p < 0.001).

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